| Autor: |
Shishkin IuL; Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Zherdev AV, Dzantiev BB, Zolotov IuA |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2000 Jul-Aug; Vol. 36 (4), pp. 497-502. |
| Abstrakt: |
A portable optoelectronic reflectometric photocolorimeter was designed. The photocolorimeter implements two operating modes (transmission and reflection), and its performance was tested in two systems of testosterone immunoenzyme assay: microplate ELISA and membrane dot-ELISA. The detection threshold for microplate ELISA and membrane dot-ELISA was 0.1 and 0.6 ng/ml, respectively. The coefficient of correlation between the photocolorimeter readings and conventional photometric methods is r = 0.999. Relative standard deviation of the results of photocolorimetric measurements (n = 4) within the optical density range from 0.03 to 1.00 ranged from 3.4 to 0.7%. Simple and versatile design of the photocolorimeter make it appropriate for testing various substances both in laboratory settings and in the field. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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