| Autor: |
Hellio Le Graverand MP; McCaig Center for Joint Injury and Arthritis Research, Faculty of Medicine, University of, Alberta, T2N 4N1, Calgary, Canada., Eggerer J, Sciore P, Reno C, Vignon E, Otterness I, Hart DA |
| Jazyk: |
angličtina |
| Zdroj: |
Matrix biology : journal of the International Society for Matrix Biology [Matrix Biol] 2000 Sep; Vol. 19 (5), pp. 431-41. |
| DOI: |
10.1016/s0945-053x(00)00093-7 |
| Abstrakt: |
The hypothesis of the present work was that expression of matrix metalloproteinase-13 (MMP-13, collagenase-3) would be induced during conditions involving important matrix remodeling such as ligament maturation, scar healing and joint instability. Therefore, MMP-13 expression in the medial collateral ligament (MCL) during the variable situations of tissue maturation and healing was assessed. MMP-13 expression in three intra-articular connective tissues of the knee (i.e. articular cartilage, menisci and synovium) following the transection of the anterior cruciate ligament of the knee was evaluated at 3 and 8 weeks post-injury. MMP-13 mRNA (semi-quantitative RT-PCR) and protein (immunohistochemistry and Western blotting) were detected in all of the tissues studied. Significantly higher MCL mRNA levels for MMP-13 were detected during the early phases of tissue maturation (i.e. 29 days in utero and 2-month-old rabbits) compared to later phases (5- and 12-month-old rabbits). This pattern of expression was recapitulated following MCL injury, with very high levels of expression in scar tissue at 3 weeks post-injury and then a decline to levels not significantly different from control values by 14 weeks. Elevated mRNA levels correlated with increased protein levels for MMP-13 in both menisci and synovium following the transection of the anterior cruciate ligament and during medial collateral ligament healing. These results indicate that MMP-13 expression is regulated by a number of variables and that high levels of expression occur in situations when connective tissue remodeling is very active. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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