CD4 T lymphocyte activation in BLV-induced persistent B lymphocytosis in cattle.

Autor: Stone DM; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, 99164-7040, USA., Norton LK, Chambers JC, Meek WJ
Jazyk: angličtina
Zdroj: Clinical immunology (Orlando, Fla.) [Clin Immunol] 2000 Sep; Vol. 96 (3), pp. 280-8.
DOI: 10.1006/clim.2000.4900
Abstrakt: Bovine leukemia virus (BLV) is an oncogenic retrovirus in the human T cell leukemia virus family. BLV infects B lymphocytes and induces a nonmalignant persistent lymphocytosis (PL) and leukemia/lymphoma in cattle. There is evidence that CD4 T lymphocytes are activated during BLV infection and promote the development of PL. How CD4 T lymphocytes are activated by BLV infection is not known. We observed that CD4 T lymphocytes from PL cattle proliferated in the presence of autologous, irradiated peripheral blood mononuclear cells (PBMC), whereas no proliferation occurred in cell cultures from BLV-infected non-PL cattle. Proliferation required direct contact with metabolically active irradiated PBMC but was not associated with viral protein expression or inhibited by antibodies to BLV. Unexpectedly, B lymphocytes alone failed to account for the irradiated PBMC stimulation of CD4 T lymphocytes. These observations and the magnitude of the proliferative response suggest that activation is polyclonal and involves mechanisms other than BLV antigen-specific stimulation.
(Copyright 2000 Academic Press.)
Databáze: MEDLINE
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