| Autor: |
Harms CA; Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA., Kennedy-Stoskopf S, Horne WA, Fuller FJ, Tompkins WA |
| Jazyk: |
angličtina |
| Zdroj: |
Fish & shellfish immunology [Fish Shellfish Immunol] 2000 Jan; Vol. 10 (1), pp. 61-85. |
| DOI: |
10.1006/fsim.1999.0230 |
| Abstrakt: |
A transforming growth factor (TGF)-beta was isolated and cloned from hybrid striped bass (Morone saxatilis x M. chrysops) anterior kidney mononuclear cells. This isolate (Genbank accession number AF140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout TGF-beta (57.3 and 78.6% identity with precursor and active protein, respectively) and rat TGF-beta 1 (41.1 and 68.8% identity with precursor and active protein, respectively). Consensus primers were demonstrated to amplify specifically by polymerase chain reaction (PCR), a TGF-beta segment from 14 species of teleost fish comprising 10 taxonomic families in 7 orders. A reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay was devised to measure TGF-beta mRNA expression in teleost fish. Higher levels of TGF-beta mRNA expression were detected in mononuclear cells of peripheral blood than from spleen or anterior kidney. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Full Text from ScienceDirect