| Autor: |
Ingkaninan K; Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands., Hazekamp A, de Best CM, Irth H, Tjaden UR, van der Heijden R, van der Greef J, Verpoorte R |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of natural products [J Nat Prod] 2000 Jun; Vol. 63 (6), pp. 803-6. |
| DOI: |
10.1021/np9905719 |
| Abstrakt: |
An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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