| Autor: |
Schmerer WM; Institute for Zoology and Anthropology, Georg-August-University Göttingen, Germany. wschmer@gwdg.de, Hummel S, Herrmann B |
| Jazyk: |
angličtina |
| Zdroj: |
Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur [Anthropol Anz] 2000 Mar; Vol. 58 (1), pp. 29-35. |
| Abstrakt: |
The analysis of degraded DNA with the help of short tandem repeat loci (STRs) is an important source of information both in forensic casework and in the anthropological context. The reproducibility of STR-genotyping of highly degraded or "ancient" DNA can be reduced by the generation of artifacts during PCR amplification. The frequency and amount of these artifacts--allelic dropout and the generation of shadow bands--are related to the quality and quantity of the extracted DNA amplified in a PCR reaction. Therefore, one important strategy to increase the reproducibility of STR-genotyping of samples containing degraded DNA is the optimisation of the DNA extraction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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