| Autor: |
Lougovskoi EV; A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv., Gogolinskaya GK |
| Jazyk: |
angličtina |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 1999 Jul-Aug; Vol. 71 (4), pp. 107-8. |
| Abstrakt: |
The active thrombin is formed in the blood stream when the blood coagulation system is activated. It attacks fibrinogen, splits off two fibrinopeptides A and fibrinogen is transformed into des-AA fibrin which is able to polymerize spontaneously forming protofibrils. At high thrombin concentration the enzyme splits off two fibrinopeptides B and des-AA fibrin units are transformed into des-AABB fibrin. These two forms of fibrin are widely used in the biological experiments. However des-AA fibrin is obtained usually from fibrinogen using the snake poisons (such as reptilase). Des-AA fibrin was obtained also by physiological enzyme thrombin, but that des-AA fibrin samples had the contamination of des-AABB fibrin. At the present paper we have described the method of the des-AA fibrin preparation by thrombin without any contamination of des-AABB fibrin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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