| Autor: |
Jacob A; Department of Obstetrics and Gynecology, North Shore University Hospital-New York University School of Medicine, Manhasset, New York 11030, USA., Hurley IR, Goodwin LO, Cooper GW, Benoff S |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular human reproduction [Mol Hum Reprod] 2000 Apr; Vol. 6 (4), pp. 303-13. |
| DOI: |
10.1093/molehr/6.4.303 |
| Abstrakt: |
Potassium (K(+)) channels are present in both mammalian testis and spermatozoa. Immunofluorescent detection of sperm-bound biotinylated charybdotoxin, an inhibitor of Ca(2+)-activated and of delayed rectifier K(+) channels, indicated that these ion channels are uniformly distributed over the surface of both heads and tails of unfixed rat epididymal spermatozoa. Reverse transcription-polymerase chain reaction (RT-PCR) analysis on rat testis RNA with PCR primers, based on known nucleotide sequences of different classes of K(+) channels, amplified sequences homologous to delayed rectifier K(+) channels. In-situ RT-PCR on rat testis sections showed that these K(+) channel transcripts are present in the cytoplasm of primary spermatocytes and post-meiotic elongating spermatids. Northern blot analysis of various rat tissues identified multiple K(+) channel transcripts, some of which were observed only in testis. An attempt to obtain a full length rat testis K(+) channel cDNA sequence gave an assembled sequence of 2693 base pairs with >90% homology to a delayed rectifier K(+) channel, Kv1.3. A method for rapid amplification of cDNA ends was employed to amplify the 5' sequences of the rat testis cDNA but a unique sequence could not be obtained. DNA sequencer traces suggest that multiple related K(+) channels which differed at their 5' ends were amplified in rat testis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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