| Autor: |
Willson VJ; Department of Physical and Metabolic Sciences, AstraZeneca R&D Charnwood, Loughborough, Leics., UK. viv.willson@charnwood.ab.astra.com, Preston JR, Lockley WJ, Mather A, Singh J, Wilkinson D |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2000 Feb; Vol. 22 (1), pp. 155-63. |
| DOI: |
10.1016/s0731-7085(99)00263-0 |
| Abstrakt: |
A radioimmunoassay has been developed for the determination of AR-C68397XX, a dual D2-receptor and beta2-adrenoceptor agonist, in human plasma. The method incorporates solid phase sample extraction and is suitable for the determination of the analyte at pg ml(-1) concentrations. The antiserum was raised in Suffolk cross sheep following primary and booster immunisations with an immunogen prepared by conjugating a carboxyphenylmethyl derivative of AR-C68397XX, to bovine serum albumin. The radioligand was prepared by the 125I-labelled iodination of a derivative of AR-C68397XX. The solid phase extraction procedure, using octadecyl sorbent, was introduced to remove matrix interferences in the plasma and to enhance method sensitivity. The calibration range is 20-500 pg ml(-1), using 0.5 ml of undiluted human plasma sample. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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