| Autor: |
Meredith GD; Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4560, USA., Sims CE, Soughayer JS, Allbritton NL |
| Jazyk: |
angličtina |
| Zdroj: |
Nature biotechnology [Nat Biotechnol] 2000 Mar; Vol. 18 (3), pp. 309-12. |
| DOI: |
10.1038/73760 |
| Abstrakt: |
We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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