| Autor: |
Agrotis A; Cell Biology Laboratory, Baker Medical Research Institute, Alfred Hospital, Prahran, Vic., Australia. alex.agrotis@baker.edu.au, Condron M, Bobik A |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 2000 Feb 04; Vol. 467 (1), pp. 128-32. |
| DOI: |
10.1016/s0014-5793(00)01132-7 |
| Abstrakt: |
We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length proteins differ from each other only by the in-frame presence or absence of Gly-Pro-Phe-Ser residues adjacent to their transmembrane domain. Stable expression of these alternate ALK-5 isoforms in ALK-5-deficient cells demonstrated that both were competent in signaling TGF-beta-induced growth inhibition and gene transcription, but with an apparently distinct potency. Our data suggest that alternate splicing within the ALK-5 gene is an important mechanism whereby SMCs may regulate their response to TGF-beta. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text