| Autor: |
Shcherbakova OG; St. Petersburg Nuclear Physics Institute, Russian Academy of Sciences. olia@omrb.pnpi.spb.ru, Smirnova TV, Filatov MV |
| Jazyk: |
ruština |
| Zdroj: |
Tsitologiia [Tsitologiia] 1999; Vol. 41 (11), pp. 946-51. |
| Abstrakt: |
A study was made of the influence of inhibitors of poly(ADP-ribose)polymerase, topoisomerase I and topoisomerase II on the frequency of gene targeting of hprt gene as well as on the frequency of random integration of targeting vector pRV9.1 into genome of mouse F9 teratocarcinoma cells. We found that the treatment of cells with the inhibitor of poly(ADP-ribose)polymerase 3-aminobenzamide after electroporation resulted in 3-4-times increase of homologous integration of exogenic vector into chromosomal DNA, and did not affect the frequency of random insertion of transfected DNA. The treatment of cells after electroporation with inhibitors of topoisomerases VP-16, ICRF-193 enhanced random integration of transfected DNA but exerted no effect on the frequency of gene targeting in this experimental system. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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