| Autor: |
Galat VV; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russia., Lagutina IS, Mezina MN, Prokof'ev MI |
| Jazyk: |
ruština |
| Zdroj: |
Ontogenez [Ontogenez] 1999 Nov-Dec; Vol. 30 (6), pp. 411-6. |
| Abstrakt: |
Successful transplantation of mammalian nuclei from differentiated cells has become possible after the application of original methods directed at the synchronization of cell cycles of the donor cell and recipient cytoplasm. We obtained a line of rabbit fetal fibroblasts which was used to study factors affecting the success of reprogramming. The nuclei of fetal fibroblasts (up to the 10th passage inclusive) proved to be capable of reprogramming and ensuring development of the cloned embryos until the preimplantation stages. The influence of synchronization of the cell cycles of the nucleus donor and recipient on the efficiency of reprogramming was studied. The rate of development of the cloned rabbit embryos to the morula-blastocyst stage reached 67% when the nuclei used were from stationary culture cells (G0-phase). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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