| Autor: |
Mulvania T; Department of Microbiology, University of Washington, Seattle, USA. mulvania@u.washington.edu, Lynch JB, Robertson MN, Greenberg PD, Morton WR, Mullins JI |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of medical primatology [J Med Primatol] 1999 Aug-Oct; Vol. 28 (4-5), pp. 181-9. |
| DOI: |
10.1111/j.1600-0684.1999.tb00268.x |
| Abstrakt: |
We describe a new surrogate assay for CD8 + T lymphocyte activity that has the capability of discriminating between cytotoxic T lymphocyte (CTL) activity and cytokine-mediated suppressive activity. We applied this approach to two groups of Macaca nemestrina vaccinated with a minimally pathogenic strain of human immunodeficiency virus type 2 [HIV-2 (HIV-2(KR))] as a model of an attenuated virus vaccine. Group 1 was then inoculated with a non-infectious stock of a pathogenic strain, HIV-2287. Both groups 1 and 2 were subsequently challenged with an infectious stock of HIV-2287. Five out of six group 1 animals were protected against CD4 decline, whereas three out of six animals in group 2 were protected. Analysis of CTL responses demonstrated strong activity against HIV-2(KR)-Gag in group 1. It was determined that strong CTL responses correlate with antigen-specific T-helper (Th) type 1 responses. This antigen-specific cytokine assay has the potential to better elucidate the functional mechanisms of CD8 + T-cell-mediated protection than traditional methods to date. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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