Valacyclovir inhibits recovery of ocular HSV-1 after experimental reactivation by excimer laser keratectomy.
| Autor: | Dhaliwal DK; Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania 15203, USA. dhaliwal@vision.eei.upmc.edu, Romanowski EG, Yates KA, Barnhorst DA, Goldstein M, Deeter RG, Fish DN, Gordon YJ |
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| Jazyk: | angličtina |
| Zdroj: | Cornea [Cornea] 1999 Nov; Vol. 18 (6), pp. 693-9. |
| DOI: | 10.1097/00003226-199911000-00012 |
| Abstrakt: | Purpose: The goal of this was to determine whether the systemic administration of valacyclovir (Valtrex) would reduce ocular shedding of herpes simplex virus 1 (HSV-1) after excimer laser ablation in the New Zealand rabbit latency model. Methods: The in vitro 50% inhibitory concentration (IC50) of HSV-1 W strain was determined by using a plaque-reduction assay to verify its sensitivity to acyclovir. Forty-seven NZW rabbits latently infected with HSV-1 W strain were divided into four groups: I, 50 mg/kg/day valacyclovir; II, 100 mg/kg/day valacyclovir; III, 150 mg/kg/day valacyclovir; and IV, saline control. One half of the total dose of valacyclovir was delivered via intraperitoneal injections twice daily for 7 days beginning with one dose before excimer laser keratectomy. HSV-1 ocular shedding was determined from eye cultures for 7 days after treatment. Results: The IC50 for HSV-1 W was determined to be 2.9 microg/ml. The administration of both 100 mg/kg/day (group II) and 150 mg/kg/day (group III) of valacyclovir significantly reduced the number of eyes from which latent HSV-1 was recovered compared with the control group. There was no difference between the control group and group I (50 mg/kg/day valacyclovir). However, all three valacyclovir dosages significantly reduced the total number of HSV-1 shedding days compared with the control group, and 100% HSV-1 TG latency was demonstrated for all four groups. Conclusion: Systemic administration of valacyclovir significantly reduced HSV-1 ocular shedding in a dose-dependent manner after excimer laser keratectomy in the NZW rabbit latency model. |
| Databáze: | MEDLINE |
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