| Autor: |
Hurrelbrink RJ; Department of Microbiology, University of Western Australia, Nedlands, WA 6907, Australia1., Nestorowicz A; Endocrine Division, Lilly Research Laboratories, Indianapolis, IN 46285, USA2., McMinn PC; Department of Microbiology, University of Western Australia, Nedlands, WA 6907, Australia1. |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of general virology [J Gen Virol] 1999 Dec; Vol. 80 ( Pt 12), pp. 3115-3125. |
| DOI: |
10.1099/0022-1317-80-12-3115 |
| Abstrakt: |
An infectious cDNA clone of Murray Valley encephalitis virus prototype strain 1-51 (MVE-1-51) was constructed by stably inserting genome-length cDNA into the low-copy-number plasmid vector pMC18. Designated pMVE-1-51, the clone consisted of genome-length cDNA of MVE-1-51 under the control of a T7 RNA polymerase promoter. The clone was constructed by using existing components of a cDNA library, in addition to cDNA of the 3' terminus derived by RT-PCR of poly(A)-tailed viral RNA. Upon comparison with other flavivirus sequences, the previously undetermined sequence of the 3' UTR was found to contain elements conserved throughout the genus FLAVIVIRUS: RNA transcribed from pMVE-1-51 and subsequently transfected into BHK-21 cells generated infectious virus. The plaque morphology, replication kinetics and antigenic profile of clone-derived virus (CDV-1-51) was similar to the parental virus in vitro. Furthermore, the virulence properties of CDV-1-51 and MVE-1-51 (LD(50) values and mortality profiles) were found to be identical in vivo in the mouse model. Through site-directed mutagenesis, the infectious clone should serve as a valuable tool for investigating the molecular determinants of virulence in MVE virus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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