| Autor: |
Hribar M; Division of Medical Intensive Care, Department of Internal Medicine, University of Geneva, Geneva, Switzerland., Bloc A, van der Goot FG, Fransen L, De Baetselier P, Grau GE, Bluethmann H, Matthay MA, Dunant Y, Pugin J, Lucas R |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of immunology [Eur J Immunol] 1999 Oct; Vol. 29 (10), pp. 3105-11. |
| DOI: |
10.1002/(SICI)1521-4141(199910)29:10<3105::AID-IMMU3105>3.0.CO;2-A |
| Abstrakt: |
Herein, we show that TNF exerts a pH-dependent increase in membrane conductance in primary lung microvascular endothelial cells and peritoneal macrophages. This effect was TNF receptor-independent, since it also occurred in cells isolated from mice deficient in both types of TNF receptors. A TNF mutant in which the three amino acids critical for the lectin-like activity were replaced by an alanine did not show any significant effect on membrane conductance. Moreover, a synthetic 17-amino acid peptide of TNF, which was previously shown to exert lectin-like activity, also increased the ion permeability in these cells. The amiloride sensitivity of the observed activity suggests a binding of TNF to an endogenous ion channel rather than channel formation by TNF itself. This may have important implications in mechanisms of TNF-mediated vascular pathology. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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