Autor: |
Yeo CB; Laser and Optical Systems Engineering Centre, Department of Mechanical Engineering, University of Glasgow, UK., Watson IA, Stewart-Tull DE, Koh VH |
Jazyk: |
angličtina |
Zdroj: |
Journal of applied microbiology [J Appl Microbiol] 1999 Sep; Vol. 87 (3), pp. 396-401. |
DOI: |
10.1046/j.1365-2672.1999.00832.x |
Abstrakt: |
Staphylococcus aureus (NCTC 6571; Oxford strain) on stainless steel discs was exposed to microwave radiation at 2450 MHz and up to 800 W. Cell viability was reduced as the exposure time increased, with complete bacterial inactivation at 110 s, attaining a temperature of 61.4 degrees C. The low rate of temperature rise, RT, of the bacterial suspension as compared with sterile distilled water or nutrient broth suggests a significant influence of the microwave sterilization efficacy on the thermal properties of the micro-organisms. The heat transfer kinetics of thermal microwave irradiation suggest that the micro-organism has a power density at least 51-fold more than its surrounding liquid suspension. When the inoculum on the stainless steel disc was subjected to microwave radiation, heat conduction from the stainless steel to the inoculum was the cause of bacteriostasis with power absorbed at 23.8 W for stainless steel and 0.16 W for the bacteria-liquid medium. This report shows that the microwave killing pattern of Staph. aureus on stainless steel was mainly due to heat transfer from the stainless steel substrate and very little direct energy was absorbed from the microwaves. |
Databáze: |
MEDLINE |
Externí odkaz: |
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