| Autor: |
Aarts LH; Rudolf Magnus Institute for Neurosciences, Department of Physiological Chemistry, Utrecht, The Netherlands., Verkade P, van Dalen JJ, van Rozen AJ, Gispen WH, Schrama LH, Schotman P |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular and cellular neurosciences [Mol Cell Neurosci] 1999 Aug; Vol. 14 (2), pp. 85-97. |
| DOI: |
10.1006/mcne.1999.0775 |
| Abstrakt: |
B-50 (GAP-43) is a neural, membrane-associated protein that has been implicated in neurite outgrowth and guidance. Following stable transfection of Rat1 fibroblasts with B-50 cDNA we observed a dispersed distribution of B-50 immunoreactivity in flattened resting cells. In contrast, motile cells exhibited high concentrations of B-50 at the leading edge of ruffling membranes, coinciding with actin polymerization. Time-lapse studies on Rat1 fibroblasts transiently transfected with B-50/EGFP revealed that large vesicles originated from the ruffling membranes. These large vesicles (pinocytes) were found positive for Thy-1, a GPI-anchored protein, but negative for rab-5, an early endosome marker. In primary hippocampal neurons B-50 also colocalized completely with the raft marker Thy-1. Antibody-mediated cross-linking of Thy-1 in hippocampal neurons resulted in a redistribution of the intracellular protein B-50 to Thy-1-immunopositive membrane patches, whereas syntaxin was mainly excluded from the patches, showing that B-50 is associated with rafts. Academic Press. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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