| Autor: |
Hanniffy OM; Department of Chemistry, National University of Ireland, Galway., Shashkov AS, Moran AP, Prendergast MM, Senchenkova SN, Knirel YA, Savage AV |
| Jazyk: |
angličtina |
| Zdroj: |
Carbohydrate research [Carbohydr Res] 1999 Jun 30; Vol. 319 (1-4), pp. 124-32. |
| DOI: |
10.1016/s0008-6215(99)00129-9 |
| Abstrakt: |
A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained beta-L-arabinose, 6-deoxy-beta-D-altro-heptose (beta-D-6d-altHep) and 6-deoxy-beta-L-altrose (beta-L-6d-Alt), whereas in the minor trisaccharide the beta-L-6d-Alt is replaced by its C-5 epimer alpha-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established: [formula: see text] |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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