| Autor: |
Meinertz JR; US Geological Survey, Upper Midwest Environmental Sciences Center, La Crosse, WI 54603, USA., Stehly GR, Hubert TD, Bernardy JA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. A [J Chromatogr A] 1999 Sep 03; Vol. 855 (1), pp. 255-60. |
| DOI: |
10.1016/s0021-9673(99)00665-2 |
| Abstrakt: |
A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., < 14%), and sensitivity (method quantitation limit, < 24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Full Text from ScienceDirect