Evaluation of three methods for hemoglobin measurement in a blood donor setting.
| Autor: | Rosenblit J; Hemotherapy Service, Hospital Israelita Albert Einstein, São Paulo, Brazil. jrosen@einstein.br, Abreu CR, Szterling LN, Kutner JM, Hamerschlak N, Frutuoso P, Paiva TR, Ferreira OC Jr |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Sao Paulo medical journal = Revista paulista de medicina [Sao Paulo Med J] 1999 May 06; Vol. 117 (3), pp. 108-12. |
| DOI: | 10.1590/s1516-31801999000300003 |
| Abstrakt: | Context: The hemoglobin (Hb) level is the most-used parameter for screening blood donors for the presence of anemia, one of the most-used methods for measuring Hb levels is based on photometric detection of cyanmetahemoglobin, as an alternative to this technology, HemoCue has developed a photometric method based on the determination of azide metahemoglobin. Objective: To evaluate the performance of three methods for hemoglobin (Hb) determination in a blood bank setting. Design: Prospective study utilizing blood samples to compare methods for Hb determination. Setting: Hemotherapy Service of the Hospital Israelita Albert Einstein, a private institution in the tertiary health care system. Sample: Serial blood samples were collected from 259 individuals during the period from March to June 1996. Main Measurements: Test performances and their comparisons were assessed by the analysis of coefficients of variation (CV), linear regression and mean differences. Results: The CV for the three methods were: Coulter 0.68%, Cobas 0.82% and HemoCue 0.69%. There was no difference between the mean Hb determination for the three methods (p>0.05). The Coulter and Cobas methods showed the best agreement and the HemoCue method gave a lower Hb determination when compared to both the Coulter and Cobas methods. However, pairs of methods involving the HemoCue seem to have narrower limits of agreement (+/- 0.78 and +/- 1.02) than the Coulter and Cobas combination (+/- 1.13). Conclusion: The three methods provide good agreement for hemoglobin determination. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|