| Autor: |
Ayar A; Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Scotland, UK., Storer C, Tatham EL, Scott RH |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroscience letters [Neurosci Lett] 1999 Aug 27; Vol. 271 (3), pp. 171-4. |
| DOI: |
10.1016/s0304-3940(99)00538-8 |
| Abstrakt: |
The whole cell patch clamp technique was used to study the effects of changes in [Ca2+]i on the excitability of cultured dorsal root ganglion (DRG) neurones. Increases in [Ca2+]i caused membrane depolarization, altered the characteristics of evoked action potentials and activated potassium, chloride and non-selective cation conductances. Mobilization of Ca2+ from intracellular stores with caffeine (1 mM) or ryanodine (10 microM) or photorelease of Ca2+ from DM-nitrophen gave rise to depolarizations suggesting a dominant inward current under our recording conditions of low [Ca2+]i buffering capacity. The actions of [Ca2+]i could be partially reversed by intracellular flash photolysis of diazo-2 but not by diazo-3. The electrophysiological properties of some DRG neurones were not influenced by changes in [Ca2+]i and we suggest that this is because in this heterogeneous culture some neurones do not express Ca2+-activated ion channels. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect