| Autor: |
Shahmolky N; Department of Clinical Biochemistry, Women's College Hospital, University of Toronto, Canada., Lefebvre DL, Poon R, Bai Y, Sharma M, Rosen CF |
| Jazyk: |
angličtina |
| Zdroj: |
Photochemistry and photobiology [Photochem Photobiol] 1999 Sep; Vol. 70 (3), pp. 341-7. |
| Abstrakt: |
Ultraviolet B radiation produces an array of cellular perturbations in the skin. We isolated a keratinocyte cDNA encoding the rat 60S ribosomal subunit protein L13a following differential cDNA library screening with UVB-enriched probes. In contrast to the reported structure of liver L13a, the keratinocyte L13a cDNA contains a longer 3'-untranslated region. Northern blot analysis detected two L13a mRNA transcripts, approximately 800 bp and approximately 1.2 kb, in keratinocytes and a variety of rat tissues. Both L13a mRNA transcripts were induced by UVB irradiation, forskolin and gamma-irradiation. In contrast, no induction of L13a mRNA transcript levels was observed following exposure of keratinocytes to 12-O-tetradecanoylphorbol-13-acetate, serum and the DNA damage-inducing agents methyl methanesulfonate or 4-nitroquinoline-N-oxide. These observations suggest that increased expression of ribosomal subunit genes may be a molecular component of the keratinocyte response to UVB in particular and not part of a nonspecific response to DNA damage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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