| Autor: |
van Dongen MJ; NSR Centre for Molecular Structure, Design, and Synthesis, Laboratory of Biophysical Chemistry, University of Nijmegen, The Netherlands., Doreleijers JF, van der Marel GA, van Boom JH, Hilbers CW, Wijmenga SS |
| Jazyk: |
angličtina |
| Zdroj: |
Nature structural biology [Nat Struct Biol] 1999 Sep; Vol. 6 (9), pp. 854-9. |
| DOI: |
10.1038/12313 |
| Abstrakt: |
H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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