| Autor: |
Comer MT; CRF Cancer Medicine Research Unit, St James's University Hospital, Leeds, UK., Shires M, Goode NP, Leese HJ, Trejdosiewicz LK, Southgate J |
| Jazyk: |
angličtina |
| Zdroj: |
The Histochemical journal [Histochem J] 1999 Jan; Vol. 31 (1), pp. 39-43. |
| DOI: |
10.1023/a:1003470113851 |
| Abstrakt: |
The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M(r) protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9 + 0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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