| Autor: |
Kwan RW; Department of Paediatrics, the University of Hong Kong, Queen Mary Hospital, Hong Kong., Wong RW, Chan SY |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of oncology [Int J Oncol] 1999 Aug; Vol. 15 (2), pp. 281-4. |
| DOI: |
10.3892/ijo.15.2.281 |
| Abstrakt: |
Epidermal growth factor (EGF) is derived from a large precursor (EGFP) of unusual structure. As EGFP remains unprocessed in certain tissues, it is of biological relevance to study its activity. Activation of the EGF receptor by EGF is involved in transformation of NIH3T3 fibroblasts. We isolated clones of NIH3T3 expressing full length, cytoplasmic region deleted or EGF-repeats deleted EGFP. All clones formed colonies in soft agarose and tumors in nude mice. Two clones expressing EGF-repeats deleted EGFP formed more and larger colonies. To conclude, EGFP is biologically active. Deletion of the 8 EGF repeats may enhance anchorage independent growth in NIH3T3. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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