| Autor: |
Reeuwijk HJ; Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands., Tjaden UR, van der Greef J |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical sciences and applications [J Chromatogr B Biomed Sci Appl] 1999 Apr 16; Vol. 726 (1-2), pp. 269-76. |
| DOI: |
10.1016/s0378-4347(99)00036-5 |
| Abstrakt: |
A capillary zone electrophoretic method for the quantification of (E)-5-(2-bromovinyl)-2'-deoxyuridine in plasma has been developed and validated. Separation was performed with a 25 mmol/l borate buffer, pH 9.0, after an initial rinsing step with sodium hydroxide. The rinsing step was necessary for reproducible analyses of aqueous samples and plasma extracts obtained by C18 solid-phase extraction after deproteination with perchloric acid. No interferences with plasma compounds were observed. The calibration graph was linear over the range of 30 to 3000 ng/ml using 5-fluorouracil as external standard. The limit of quantification was 24 ng/ml. The CZE method is fast, reproducible, linear and is therefore a good alternative for the already established HPLC methods. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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