Virus passage through track-etch membranes modified by salinity and a nonionic surfactant.

Autor: Lytle CD; Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20857, USA. cdl@cdrh.fda.gov, Routson LB, Jain NB, Myers MR, Green BL
Jazyk: angličtina
Zdroj: Applied and environmental microbiology [Appl Environ Microbiol] 1999 Jun; Vol. 65 (6), pp. 2773-5.
DOI: 10.1128/AEM.65.6.2773-2775.1999
Abstrakt: Why do viruses sometimes not pass through larger pores in track-etch filters? Increasing the salinity (0.8 to 160 mM Na+) decreased phiX174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyester membranes. Undiminished passage when 0.1% Tween 80 was added implied that nonionic virus adsorption occurred and indicated that high levels of salinity decreased virus passage by decreasing electrostatic repulsion that prevented adsorption.
Databáze: MEDLINE