| Autor: |
Mittal SK; Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907-1243, USA. skmittal@vet.purdue.edu, Tikoo SK, van den Hurk JV, Breker-Klassen MM, Yoo D, Babiuk LA |
| Jazyk: |
angličtina |
| Zdroj: |
Intervirology [Intervirology] 1998; Vol. 41 (6), pp. 253-60. |
| DOI: |
10.1159/000024947 |
| Abstrakt: |
We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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