| Autor: |
Reichner JS; Department of Surgery, Division of Surgical Research, Brown University and Rhode Island Hospital, Providence, Rhode Island 02903, USA. Jonathan_Reichner@brown.edu, Meszaros AJ, Louis CA, Henry WL Jr, Mastrofrancesco B, Martin BA, Albina JE |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of pathology [Am J Pathol] 1999 Apr; Vol. 154 (4), pp. 1097-104. |
| DOI: |
10.1016/S0002-9440(10)65362-X |
| Abstrakt: |
Tissue injury initiates a temporally ordered sequence of local cellular and metabolic responses presumably necessary for successful repair. Previous investigations demonstrated that metabolic evidence for nitric oxide synthase (NOS) activity is detectable in wounds only during the initial 48 to 72 hours of the repair process. Present results identify the cell types contributing inducible NOS (iNOS) to experimental wounds in rats. iNOS antigen was expressed in most macrophages present in wounds 6 to 24 hours after injury, and these cells exhibited NAPDH diaphorase and NOS activity. Polymorphonuclear leukocytes contained little iNOS antigen and no NADPH diaphorase activity and were minimally able to convert L-arginine to L-citrulline. The frequency of iNOS-positive macrophages declined on days 3 and 5 after wounding. By day 10, most macrophages in the wound were negative for iNOS. These cells, however, acquired iNOS antigen and activity in culture. Wound fluids, but not normal rat serum, suppressed the induction of iNOS during culture. Findings indicate that the expression of iNOS in healing wounds is restricted to macrophages present during the early phases of repair and that components of wound fluid suppress the induction of iNOS in macrophages in late wounds. Polymorphonuclear leukocytes contribute little iNOS activity to the healing wound. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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