| Autor: |
Vatcher GP; Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada., Barbazuk WB, O'Neil NJ, Marra MA, Ha T, Baillie DL |
| Jazyk: |
angličtina |
| Zdroj: |
Gene [Gene] 1999 Apr 16; Vol. 230 (2), pp. 137-44. |
| DOI: |
10.1016/s0378-1119(99)00076-1 |
| Abstrakt: |
In the nematode Caenorhabditis elegans, the maternal effect lethal gene mel-32 encodes a serine hydroxymethyltransferase isoform. Since interspecies DNA comparison is a valuable tool for identifying sequences that have been conserved because of their functional importance or role in regulating gene activity, mel-32(SHMT) genomic DNA from C. elegans was used to screen a genomic library from the closely related nematode Caenorhabditis briggsae. The C. briggsae genomic clone identified fully rescues the Mel-32 phenotype in C. elegans, indicating functional and regulatory conservation. Computer analysis reveals that CbMEL-32(SHMT) is 92% identical (97% similar) to CeMEL-32(SHMT) at the amino acid level over the entire length of the protein (484 amino acids), whereas the coding DNA is 82.5% identical (over 1455 nucleotides). Several highly conserved non-coding regions upstream and downstream of the mel-32(SHMT) gene reveal potential regulatory sites that may bind trans-acting protein factors. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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