| Autor: |
Barchiesi F; Institute of Infectious Diseases and Public Health of the University of Ancona, Italy. cmalinf@popcsi.unian.it, Arzeni D, Compagnucci P, Di Francesco LF, Giacometti A, Scalise G |
| Jazyk: |
angličtina |
| Zdroj: |
Medical mycology [Med Mycol] 1998 Dec; Vol. 36 (6), pp. 437-40. |
| DOI: |
10.1080/02681219880000701 |
| Abstrakt: |
We evaluated the in vitro activity of fluconazole, itraconazole, ketoconazole, 5-fluorocytosine and amphotericin B against 30 clinical isolates of Saccharomyces cerevisiae by a broth microdilution method, following the NCCLS recommendation. Testing was performed either in RPMI-1640 or yeast nitrogen base (YNB). YNB supported the growth of all isolates tested, while results in RPMI-1640 were not obtained for six isolates (20%). The MIC of all three azoles in YNB were one or two dilutions higher than those obtained in RPMI-1640 (P=0.0001 for fluconazole and itraconazole, P=0.03 for ketoconazole). Elevated MICs were observed for all three azoles, while all the isolates were susceptible to 5-fluorocytosine and amphotericin B. All MIC values were confirmed by spectrophotometric reading. Six strains of S. cerevisiae isolated from the faeces and consecutive blood cultures from an AIDS patient over a 7-month period were typed by electrophoretic karyotyping (EK). EK showed the maintenance of the same karyotype over time suggesting that the faecal isolate changed from a colonizing to infection-causing strain. The relative resistance of S. cerevisiae to azole drugs as well as its ability to cause widespread infections may promote the emergence of this species as a pathogen in immunosuppressed patients. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text