| Autor: |
Pochkhidze MSh; Department of Chemistry, Tbilisi State University, Republic of Georgia., Asatiani LP, Rukhadze MD, Chitiashvili ZJ, Tsartsidze MA |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical chromatography : BMC [Biomed Chromatogr] 1999 Feb; Vol. 13 (1), pp. 89-91. |
| DOI: |
10.1002/(SICI)1099-0801(199902)13:1<89::AID-BMC812>3.0.CO;2-8 |
| Abstrakt: |
Ferrocene-A (ferrocenyl-1-phenyl-1-dioxy-1,4-butin-2) has shown significant antitumour and antibacterial activity. To facilitate studies in this field a simple and rapid liquid chromatographic procedure was developed, allowing the determination of ferrocene-A in the blood serum. The procedure was based on a reversed-phase high-performance liquid chromatography (HPLC) method, with UV detection at 210 nm. The mobile phase ethanol-0.01 M KH2PO4 60:40 (v/v) provides the complete separation of ferrocene-A, internal standard and endogenous compounds of serum. The recovery of ferrocene-A from serum using a hexaneisoamyl alcohol mixture was 90%. The proposed method is convenient for pharmacological and pharmacokinetic applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Plný text