Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the fluorometric measurement of fatty acid concentrations.

Autor: Demant EJ; Biochemistry Laboratory C, Panum Institute, University of Copenhagen, Copenhagen, DK-2200, Denmark.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 1999 Feb 15; Vol. 267 (2), pp. 366-72.
DOI: 10.1006/abio.1998.3019
Abstrakt: A covalent complex between bovine serum albumin and 7-hydroxycoumarin-4-acetic acid (BSA-HCA) shows a strong fluorescence band at lambdamax = 450 nm upon excitation at 375 nm. Quenching of the fluorescence emission accompanies the association of fatty acids (FA) to BSA-HCA and the application of the complex as a spectrofluorometric probe for measurement of fatty acid concentrations in aqueous solution is examined. Binding constants for various long-chain fatty acids (Kd = 14-460 nM) and calibration curves characterizing the probe have been determined. Standardized assay conditions allow for accurate measurements in the concentration range of 10 nM to 5 microM. BSA-HCA provides a stable and sensitive fluorescence-based FA probe with potential biochemical applications.
(Copyright 1999 Academic Press.)
Databáze: MEDLINE
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