Effect of gamma-radiation on the growth of primary and "long-term" tissue cultures (Nicotiana tabacum L.)

Autor: Opatrný, Zdeněk, 1941-
Jazyk: angličtina
Předmět:
Druh dokumentu: Non-fiction
Abstrakt: Abstract: Growth of primary (cv. Wisconsin 38) and long-term (cv. Virginia Bright Italia) tissue cultures obtained from Nicotiana tabacum L. pith was affected by exposure to a single dose of gamma-radiation ranging from 1 to 150 krad. The inhibitory effect was proportional to the applied dose. Changes in the degree of inhibition during the subculture interval were ascertained by quantitative evaluation of the growth response. Primary cultures proved to be several times more radiosensitive than long-term cultures, the capacity to recover from radiation damage of the cultures participating in the resulting manifestation of the effect. The possible reasons for these differences are discussed.
Databáze: Katalog Knihovny AV ČR