Effect of EDTA on transport forms of manganese in maize xylem exudate.
| Autor: | Ebeid, M. M. |
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| Další autoři: |
Kutáček, Milan, 1925-1989
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| Jazyk: | angličtina |
| Předmět: | |
| Druh dokumentu: | Non-fiction |
| Abstrakt: | Abstract: Xylem exudates were collected from 20-day-old maize seedlings grown for 24 h in labelled manganese chloride solution (10 ppm) with or without EDTA (18 mgl-1). The distribution of radioactivity on paper ohromatograms of exudates did not show the existence of free Mn. Fractionation of exudate constituents by gel filtration on Sephadex G-26 showed that 54Mn is associated with three UV (254 nm) - absorbing fractions. Two fractions contain amino acids (M. wt.:>=10 000 and ~ 900). The third fraction (M. wt. ~ 600) however, appears to be a carrier of a different type, which is practically amino acid free, although it contains 10.23% N (by elementary analysis). The properties of this carrier are discussed. Application of EDTA has favoured the; transport of Mn in xylem sap with fractions of relatively lower molecular weights. |
| Databáze: | Katalog Knihovny AV ČR |
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