A BamHI family of tobacco highly repeated DNA : a study about its species-specificity.

Autor: Koukalová, Blažena, 1935-
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Jazyk: angličtina
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Druh dokumentu: Non-fiction
ISSN: 0006-3134
Abstrakt: Abstract: A BamHI family of highly repeated DNA sequences of theNicotiana tabacum nuclear genome, denoted as a HRS60-family, was recently isolated. It comprises about 2% of the tobacco nuclear genome. Monomeric units are 182-184 bp long. Members of the HRS60-family isolated till now are closely related. DNA-DNA hybridization experiments with DNA of the two tobacco progenitors, N. tomentosiformis and N. sylvestris, revealed that the HRS60-family was present in many copies inN. sylvestris, the amount being about 1.7 times that inN. tabacum. InN. tomentosiformis as well as in some other species of the genusNicotiana, the HRS60-family is present in a small amount. Sequences related to the HRS60-family were revealed using DNA-DNA hybridization at low stringency. With respect to quantity, the HRS60-family could be considered as a species-specific DNA repeat which may be a useful genetic marker in genetic manipulations with N. tabacum.
Databáze: Katalog Knihovny AV ČR
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