The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria.

Autor:	Koubová, Anna
Další autoři:	Knapp, Brigitte A. Insam, Heribert, 1957- Pižl, Václav, 1956- Elhottová, Dana
Jazyk:	angličtina
Předmět:	půdní zoologie půdní mikrobiologie archea bakterie žížalovití kroužkovci soil zoology soil microbiology Archaea bacteria Lumbricidae Annelida články journal articles mikrobiální rozmanitost žížala hnojní žížala kalifornská průchod střev PCR-DGGE molekulární otisky prstů microbial diversity Eisenia fetida Eisenia andrei gut passage molecular fingerprinting
Druh dokumentu:	Non-fiction
ISSN:	1211-376X
Abstrakt:	Abstract: The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forest soil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of Eisenia andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of Eisenia fetida and E. andrei, respectively, had a significant effect on the composition of the archaeal community. Shifts in the composition of the compost bacterial community were more apparent after passage through the guts Eisenia andrei than of E. fetida. This study is the first on the changes in the composition of the archaeal community in substrates that occur during their passage through the guts of two species of Eisenia.
Databáze:	Katalog Knihovny AV ČR
Externí odkaz:	Plný text