| Abstrakt: |
Abstract: Hydrogen peroxide is an effective abiotic elicitor that can induce secondary metabolite biosynthesis in plants. We show that in cell suspension culture of a salt-tolerant medicinal plant Cistanche salsa, the production of bioactive components phenylethanoid glycosides (PeGs) was increased after an H2O2 treatment. To identify genes related to PeGs biosynthesis affected by H2O2, we constructed a suppression subtractive hybridization library of H2O2 responsive genes using a C. salsa cell line and identified 105 expressed sequence tags (ESTs) and 85 genes. EST library functional annotation and gene ontology analyses showed genes related to various stress responses, biosynthesis of secondary metabolites, and transcriptional regulation. Among them we identified two genes related to the PeGs biosynthesis pathway (4-coumarate coenzyme A ligase and cinnamate 4-hydroxylase), and two WRKY type transcription factors. The expressions of selected genes after the H2O2 treatment were analyzed by RT-qPCR. An early increased transcription of PeG biosynthesis pathway genes after the treatment revealed that H2O2 induced PeGs biosynthesis via up-regulation of its key genes. |
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