CD36 regulates fatty acid composition and sensitivity to insulin in 3T3-L1 adipocytes.

Autor:	Kontrová, K.
Další autoři:	Zídková, Jarmila, 1952- Bartoš, B. Skop, V. Sajdok, Jiří Kazdová, Ludmila, 1938- Mikulík, K. Mlejnek, Petr Zídek, Václav, 1951- Pravenec, Michal, 1953-
Jazyk:	angličtina
Předmět:	fyziologie mastné kyseliny inzulin physiology fatty acids insulin články journal articles CD36 3T3-L1 adipocyty interference RNA 3T3-L1 adipocytes RNA interference
Druh dokumentu:	Non-fiction
ISSN:	0862-8408
Abstrakt:	Abstract: In the current study, we tested a hypothesis that CD36 fatty acid (FA) transporter might affect insulin sensitivity by indirect effects on FA composition of adipose tissue. We examined the effects of CD36 downregulation by RNA interference in 3T3-L1 adipocytes on FA transport and composition and on sensitivity to insulin action. Transfected 3T3-L1 adipocytes, without detectable CD36 protein, showed reduced neutral lipid levels and significant differences in FA composition when levels of essential FA and their metabolites were lower or could not be detected including gamma linolenic (C18:3 n6), eicosadienic (C20:2 n6), dihomo-gamma linolenic (C20:3 n6), eicosapentaenoic (EPA) (C20:5 n3), docosapentaenoic (DPA) (C22:5 n3), and docosahexaenoic (DHA) (C22:6 n3) FA. Transfected 3T3-L1 adipocytes exhibited a significantly higher n6/n3 FA ratio, reduced Δ5-desaturase and higher Δ9-desaturase activities. These lipid profiles were associated with a significantly reduced insulin-stimulated glucose uptake (4.02±0.1 vs. 8.42±0.26 pmol.10-3 cells, P=0.001). These findings provide evidence that CD36 regulates FA composition thereby affecting sensitivity to insulin action in 3T3-L1 adipocytes.
Databáze:	Katalog Knihovny AV ČR
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