Differential regulation of galectin expression/reactivity during wound healing in porcine skin and in culture of epidermal cells with functional impact of migration.
| Autor: | Klíma, Jiří |
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| Další autoři: |
Lacina, Lukáš, 1979-
Dvořánková, Barbora, 1955-
André, Sabine, 1966-
Motlík, Jan, 1946-
Gabius, Hans-Joachim, 1955-
Smetana, Karel, 1958-
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| Jazyk: | angličtina |
| Předmět: | |
| Druh dokumentu: | Non-fiction |
| Abstrakt: | Abstract: The glycophenotyping of mammalian cells with plant lectins maps aspects of the glycomic profile and disease-associated alterations. A salient step toward delineating their functional dimension is the detection of endogenous lectins. They can translate sugar-encoded changes into cellular responses. Among them, the members of the lectin family of galectins are emerging regulators of cell adhesion, migration and proliferation. Focusing on galectins-1, -3 and -7, we addressed the issue whether their expression is regulated during wound healing in porcine skin as model. A conspicuous upregulation is detected for galectin-1 in the dermis and a neoexpression in the epidermis, where an increased level of galectin-7 was also found. Applying biotinylated tissue lectins as probes, the signal intensities for accessible binding sites decreased, intimating an interaction of the cell lectin with reactive sites. In contrast, galectin-3 parameters remained rather constant. Of note, epidermal cells in culture also showed an increase in expression/presence of galectin-1, measured on the levels of mRNA and protein, in this case by Western blotting and quantitative immunocytochemistry. Used as matrix, galectin-1 conferred resistance to trypsin treatment to attached human keratinocytes and reduced migration into scratch-wound areas in vitro. This report thus presents new information on endogenous lectins in wound healing and differential regulation among the three tested cases. |
| Databáze: | Katalog Knihovny AV ČR |
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