| Abstrakt: |
Abstract: The effects of abiotic stresses on the expression of Lhcb1 gene (coding light-harvesting chlorophyll-protein complex II), and on photosynthetic rate were studied in one Oenanthe javanica (cv. Baguazhou Shuiqin) and three Apium graveolens cultivars (Liuhe Huangxinqin, Jinnan Shiqin, and Ventura). The Lhcb1 genes were cloned and we predicted that Lhcb1 proteins were most probably able to form homo-trimers. Each monomer contained five helical segments, of which three were likely transmembrane helices, and 15 putative chlorophyll-binding sites. The abiotic stresses affected the A. graveolens similarly in all the cultivars, however, the O. javanica photosynthesis was not significantly affected. The expression of the Lhcb1 gene was up-regulated under the cold, heat, salt, and drought stresses in the cvs Liuhe Huangxinqin and Jinnan Shiqin. The Lhcb1 was up-regulated under the heat, salt, and drought stresses in the cv. Ventura, whereas had no significant changes under the cold stress. No significant changes of the Lhcb1 expression were found under the cold and salt stresses and even little down-regulation following the heat and drought stresses in Oenanthe javanica. The expression of Lhcb1 may be a useful indicator of photosynthetic activity. |
