| Abstrakt: |
Abstract: Phenylalanine ammonia lyase (PAL) is a key enzyme in the phenylpropanoid pathway responsible for biosynthesis of many secondary metabolites, such as anthocyanins, flavanols, and lignins. The objective of this work was to determine the effect of different rootstock/scion combinations on Prunus tree growth, PAL gene expression, and PAL activity in order to identify compatibility of different graft combinations. The study was performed with peach (P. persica cv. Chimarrita) grafted on two peach rootstocks (Capdeboscq and Tsukuba1) and one Japanese apricot (P. mume cv. Umezeiro). Two or three years after grafting, the growth of peach scion on Umezeiro was weak and finally the death of some trees occurred. The peach rootstocks induced vigorous growth without any tree loss through the three years of evaluation. However, PAL activity and expression of PAL encoding genes were higher in Umezeiro as compared to the other rootstocks. These results show that the differential gene expression together with the PAL activity is a promising strategy to predict graft incompatibility. |
