Time-lapse cleavage rating predicts human embryo viability.

Další autoři:	Hlinka, Daniel Kal'atová, B. Uhrinová, I. Dolinská, S. Rutarová, J. Řezáčová, Jitka, 1958- Lazarovská, Sonja Dudáš, M.
Jazyk:	angličtina
Předmět:	fyziologie člověka human physiology články journal articles time-lapse imaging in human embryos cell cycle analysis trichotomic / Tripolar mitosis selection for single-embryo transfer preimplantation screening and diagnosis clinical cytometry and morphometry
Druh dokumentu:	Kniha
Abstrakt:	Abstract: Chronology of three consecutive mitotic events in human preimplantation embryos was examined by time-lapse imaging. In zygotes producing well-formed and pregnancy-yielding expanded blastocysts, uniform time-patterning of cleavage clusters (c) and interphases (i) was revealed: i2=11±1, i3=15±1, i4=23±1 h / c2=15±5, c3=40±10, c4=55±15 min. Oppositely, shortened or prolonged durations of one or more cell cycles were strongly predictive of poor implantation and development. Furthermore, trichotomic mitosis was discovered in 17 % of cases - zygotes cleaved into 3 blastomeres and 2-cell embryos into 5-6 cells (instead of normal 2 and 4). During conventional clinical assessment, such embryos are indistinguishable from normal, often considered just-in-course of the next cell cycle. Only detailed time-lapse monitoring paced at 10-minute intervals had proven all these embryos to be absolutely unviable, even in rare cases when they reduced their hypercellularity to normal cell counts via cell-cell fusion. Overall, we demonstrate that timelapse embryo cleavage rating (ECR) as a standalone diagnostic procedure allows for effective identification of viable early embryos with 90 % specificity, while elimination of good-looking but unviable embryos can be assumed with a specificity of 100 %. Thus, making this non-invasive and contactless approach worth of addition to routine embryo screening in clinical IVF programs.
Databáze:	Katalog Knihovny AV ČR
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