| Abstrakt: |
Abstract: Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived fragments (TDFs) were found to be up-regulated and 17 down-regulated during chilling stress. Eighteen TDF of up-regulated genes were cloned and sequenced. Some of these genes are involved in the stress response, some play important roles in energy and substrate metabolism, and some encode unknown proteins such as TDF119. Two sequences, designated TDF217 and TDF223, may correspond to novel genes. The expression profiles of 6 from 18 TDFs were analyzed by quantitative real-time PCR under chilling and abscisic acid (ABA) stress. It was demonstrated that all 6 genes were significantly induced by chilling and their expression was decreased when the temperature was shifted from 4 to 25 °C. The transcriptional levels of 5 TDFs were strongly enhanced also in response to exogenous ABA. Based on the characteristics of genes isolated from seedlings exposed to cold stress, we conclude that Lepidium adapts to cold stress by regulating many signal transduction pathways, including both ABA-dependent and ABA-independent signaling pathways. |
