| Autor: |
Wang, Haihua, You, Baorong, Miao, Qing, Liu, Bingci, Zhou, Peihong, Fu, Dechen, Guan, Ran |
| Předmět: |
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| Zdroj: |
Journal of Environmental Pathology, Toxicology & Oncology. 2000, Vol. 19 Issue 1/2, p1. 0p. |
| Abstrakt: |
Crystalline silica was recently classified as a human carcinogen by the International Agency for Research on Cancer (IARC). However, the direct genotoxic effect of silica in humans remains unclear. We examined the p53 and K-ras gene mutations in lung cancer in workers with silicosis (LCWS). DNA was extracted from paraffin-embedded tissues andexamined by PCRRFLP, PCR-SSCP, and DNA sequencing. The mutation frequencies of p53 gene were high, but the mutation distributions in exons and among the histological types of LOWS differed from those of common (i.e., not silicosisrelated) lung cancer. Furthermore, no mutations in codon 12 of K-ras gene (predominant in common lung cancer) werefound in LOWS. These findings in the mutational spectrum support a carcinogenic effect of silica dust at the DNA molecular level. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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