| Autor: |
Yi, Liu, Zhi-qun, Tan, Tian-zhi, Wang, Ping, Shen, Cheng-nong, Yan, Song-sheng, Qu |
| Předmět: |
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| Zdroj: |
Chemosphere. Feb1999, Vol. 38 Issue 4, p891. 0p. |
| Abstrakt: |
By using a LKB2277 BioActivity Monitor (heat conduction rnicrocalorimetcr), stopped-flow method, the thermogenetic curves of Rhizopus nigricans growth at 25 IC inhibited by four kinds of heavy metal ions are determined, parameters such as growth rate constants k, inhibitory ratio I, half inhibitory concentration IC50 et al. are obtained. The experimental results show that heavy metal ions can inhibit Rhizopus nigricans growth obviously, low concentration of Cu"' has promoting action. The inhibitory sequence is Cd2+ > Hg2+ >Pb2+ > CU2+, half inhibitory concentration of them are Cd2+ 0.8 mu g.ml-1, Hg2+ 1.7 mu g.ml-1, Pb2+ 48.0 mu g.ml-1 , Cu2+ 110 mu g.ml-1. This microclorimetric bioassay for acute cellular toxicity is based on metabolic heat evolution from cultured cells. Ile assay is quantitative, inexpensive, and versatile; moreover toxicological information can be obtained with cell from other species of interest. (c) 1998 Elsevier Science Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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