In vitro clastogenicity and phototoxicity of fullerene (C60) nanomaterials in mammalian cells

Autor:	Honma, Masamitsu1 honma@nihs.go.jp, Takahashi, Toshitaka2, Asada, Shin2, Nakagawa, Yuzuki2, Ikeda, Atsuko2, Yamakage, Kohji2
Předmět:	*Mutagens Fullerenes Nanostructured materials Cytokinesis Titanium dioxide DNA damage Chromosome abnormalities Graphene Carbon nanotubes Cell lines
Zdroj:	Mutation Research - Genetic Toxicology & Environmental Mutagenesis. Dec2012, Issue 1/2, p97-100. 4p.
Abstrakt:	Abstract: Carbon nanomaterials such as carbon nanotubes, graphene, and fullerenes (C60) are widely used in industry. Because of human health concerns, their toxic potential has been examined in vivo and in vitro. Here we used mammalian cells to examine the in vitro clastogenicity as well as the phototoxicity of C60. While C60 induced no structural chromosome aberrations in CHL/IU cells at up to 5mg/ml (the maximum concentration tested), it significantly induced polyploidy at 2.5 and 5mg/ml with and without metabolic activation. In BALB 3T3 cells, C60 showed no phototoxic potential but the anatase form of titanium oxide did. Since insoluble nanomaterials cause polyploidy by blocking cytokinesis rather than by damaging DNA, we concluded that the polyploidy induced by C60 in CHL/IU cells was probably due to non-DNA interacting mechanisms. [Copyright &y& Elsevier]
Databáze:	GreenFILE
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