Inhibitory action of Cerrado plants against mammalian and insect α-amylases

Autor: Silva, Everton M.1, Valencia, Arnubio1,2,3, Grossi-de-Sá, Maria Fátima2,4, Rocha, Thales L.2, Freire, Érika4,5, de Paula, José E.6, Espindola, Laila S.1 darvenne@unb.br
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Zdroj: Pesticide Biochemistry & Physiology. Nov2009, Vol. 95 Issue 3, p141-146. 6p.
Abstrakt: Abstract: A total of 185 hexanic, dichloromethanic, ethanolic and hydroethanolic extracts from 24 species of Cerrado plants, were tested against Zabrotes subfasciatus, Acanthoscelides obtectus, and human saliva α-amylases. Twelve crude extracts presented inhibition rates greater than 80% against digestive α-amylases of the insect pest Z. subfasciatus, at a concentration of 1mgmL−1. These extracts were also tested against A. obtectus and human saliva α-amylases to verify their affinity and specificity of action. The hydroethanolic Kielmeyera coriacea stem bark extract presented a strong inhibitory potential, with IC50 values of 110μgmL−1 for Z. subfasciatus and 272.12μgmL−1 for A. obtectus, in addition to a 97.09% reduction in enzyme activity of human saliva α-amylases at 125μgmL−1. The hexanic Aspidosperma macrocarpon root wood extract totally inhibited the activity of Z. subfasciatus α-amylases, reduced the enzyme activity of A. obtectus by 14.69% at 1mgmL−1, but did not alter the activity of human saliva α-amylases, thus characterizing greater inhibition affinity and specificity. The results suggest that the application of plant extracts against insect α-amylases represent a promising biotechnological tool for development of new insect pest control strategies, with noticeable affinity and specificity of action against different target enzymes. [Copyright &y& Elsevier]
Databáze: GreenFILE
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